Research Associate, Cell Culture Process DevelopmentLocation Novato, California Apply
BioMarin is the world leader in delivering therapeutics that provide meaningful advances to patients who live with serious and life-threatening rare genetic diseases. We target diseases that lack effective therapies and affect relatively small numbers of patients, many of whom are children. These conditions are often inherited, difficult to diagnose, progressively debilitating and have few, if any, treatment options. BioMarin will continue to focus on advancing therapies that are the first or best of their kind.
BioMarin’s Cell Culture Process Development groupis responsible for designing and scaling up the cell culture processes for production of biomolecules, including proteins for enzyme replacement therapies and viral vectors for gene therapies.
The Research Associate will be part of a stablished team of scientists and RAs working to develop and optimize AAV vectors production processes and assists team members in molecular biology and cell culturetasks including:
- Nucleic acid/plasmid purification.
- Cell propagation and plasmid transfection.Clone screening and selection.
- DNA analysis by agarose electrophoresis. PCR.
- DNA quantification by qPCR or ddPCR.
- Protein analysis by ELISA, western blot.
- Thorough documentation of procedures and results.
- Flexibility, willingness to accept new responsibilities and perform in a fast paced environment.
- Handle multiple tasks efficiently.
- Closely interact with other team members; develop internal and external collaborations.
- Present results and conclusions in group and department meetings.
- Molecular biology and cell culture laboratory work experience.
- Media and reagents preparation.
- Good organization, data analysis and interpretation skills.
- Critical thinking, good interpersonal, verbal, and written communication skills.
- Proficiency in Microsoft Word, Excel, and PowerPoint.
- DNA/RNA purification. In vitro DNA modification/Recombinant DNA techniques.
- Hands on experience with Adeno-associated virus (AAV) vectors.
- Analytical assays: qPCR, ddPCR, ELISA, electrophoresis, Western blot, cell-based assays.
Minimum: Bachelor’s degree in biological sciences or in a related field with a concentration in molecular biology.
Preferred: Bachelor’s degree with 2+ years or Master’s degree with 1+ years of laboratory experience in molecular biology and cell culture.
We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.